Journal: Respiratory Research

Article Title: Blood oxygen regulation via P2Y12R expressed in the carotid body

doi: 10.1186/s12931-024-02680-x

Figure Lengend Snippet: Physiological parameters of control groups preceding hypoxic challenge

Article Snippet: ARL 67156 (Cat. No. 1283; PubChem CID: 11957464), Clopidogrel hydrogen sulfate (Cat. No. 1820; PubChem CID: 115366) and PSB 0739 (Cat. No. 3983; PubChem CID: 44583582) were from Tocris Biosciences, Bristol, UK.

Techniques: Control

Journal: Respiratory Research

Article Title: Blood oxygen regulation via P2Y12R expressed in the carotid body

doi: 10.1186/s12931-024-02680-x

Figure Lengend Snippet: Physiological parameters of experimental groups preceding hypoxic challenge

Article Snippet: ARL 67156 (Cat. No. 1283; PubChem CID: 11957464), Clopidogrel hydrogen sulfate (Cat. No. 1820; PubChem CID: 115366) and PSB 0739 (Cat. No. 3983; PubChem CID: 44583582) were from Tocris Biosciences, Bristol, UK.

Techniques:

Journal: Respiratory Research

Article Title: Blood oxygen regulation via P2Y12R expressed in the carotid body

doi: 10.1186/s12931-024-02680-x

Figure Lengend Snippet: Glomic P2Y12R, but not centrally expressed receptors or thrombocytes influence chemoreflex in vivo. A – I Experimental animals were treated with clopidogrel (10 mg/kg i.p., 60 min prior to the experiment) ( A – C ); PSB 0739 (0.3 mg/kg i.t., 18 h prior to the experiment) ( D – F ); the mouse specific anti-CD41 antibody (deplete thrombocytes) ( G – I ) or the respective vehicle; and mean arterial pressure, heart rate, oxygen saturation and respiratory rate were recorded during ingravescent hypoxic challenge. Distinct stages of hypoxic environmental challenges are indicated with colors: normoxia ( light blue ), mild hypoxia ( yellow ) severe hypoxia ( red ) and recurrent normoxia (normoxia II., dark blue ). Data represent the mean ± SD. Box-and-whisker diagrams demonstrate statistical differences between control and treatment groups during the identical challenge stage. Data show the median, the minimum and maximum values, and the interquartile range (n = 11 for Clopidogrel; n = 12 for PSB 0739; and n = 10 for platelet depleted) ( A , D , G ). Arterial blood-gas and pH changes are shown for clopidogrel (n = 9) (B), PSB 0739 (n = 8) ( E ) and platelet depleted groups (n = 10) ( H ) compared to the respective control animals (n = 9 for Control (clopidogrel); n = 12 Control (PSB 0739); and n = 10 Control (depleted) groups). Graphs compare the area under curve (AUC) values of breath distension for the clopidogrel (n = 7) ( F ) and platelet depleted experimental groups (n = 5–8) ( I ). *, p ≤ 0.05 [two-way ANOVA with Bonferroni’s post-hoc test ( A , D , G ); one-way ANOVA with Tukey’s post-hoc test ( B , E , H ) and unpaired two-tailed Student’s t -test ( C , F , I )]

Article Snippet: ARL 67156 (Cat. No. 1283; PubChem CID: 11957464), Clopidogrel hydrogen sulfate (Cat. No. 1820; PubChem CID: 115366) and PSB 0739 (Cat. No. 3983; PubChem CID: 44583582) were from Tocris Biosciences, Bristol, UK.

Techniques: In Vivo, Whisker Assay, Control, Two Tailed Test

Journal: Cell reports

Article Title: Microglia enable mature perineuronal nets disassembly upon anesthetic ketamine exposure or 60-Hz light entrainment in the healthy brain

doi: 10.1016/j.celrep.2021.109313

Figure Lengend Snippet: (A-C) Microglia depletion with Csf1r-inhibitor PLX5622 and 3× saline or KXA ketamine treatment. (A) Experimental timeline. (B) Immunostaining for WFA. Scale bar: 200 μm. Next, zoom-in into S1. Scale bar: 20 μm. (C) Mean density of PNN-coated cells ± SEM. Grey and green dashed lines: reference for mean value of 3× saline or KXA, respectively, from Figures 1C and S1D. Two sample t-test: P= 0.3467. (D-G) Clopidogrel treatment, i.p. injected alone (3× clopidogrel) or 5 min before KXA application (3× (clopidogrel + KXA)). (D) Experimental timeline. (E) Immunostaining for WFA. Scale bar: 200 μm. (F) Mean density of PNN-coated cells ± SEM. Grey and green dashed lines: reference for mean value of 3× saline or 3× KXA, respectively, from Figures 1C and S1D. Two sample t-test: P=0.3808. Each dot represents an animal. (G) Higher magnification images for WFA (magenta or contrast-image) and Iba1 (green). Scale bar: 30 μm. nsP > .05.

Article Snippet: Clopidogrel: Clopidogrel (Tocris, Cat#2490) was dissolved in dimethyl sulfoxide (DMSO, Sigma, Cat#D8418) and diluted 1:4 in phosphate buffer saline (PBS) before the injection.

Techniques: Saline, Immunostaining, Injection

Journal: Cell

Article Title: RESIDENT MACROPHAGES CLOAK TISSUE MICROLESIONS TO PREVENT NEUTROPHIL-DRIVEN INFLAMMATORY DAMAGE

doi: 10.1016/j.cell.2019.02.028

Figure Lengend Snippet: KEY RESOURCE TABLE

Article Snippet: Inhibitor studies Macrophages were topically pre-treated with following inhibitors at indicated concentrations: list-behavior=unordered prefix-word= mark-type=disc max-label-size=0 Apyrase (Sigma-Aldrich; Cat#A6535): H20; 10 U MRS2578 (Tocris; Cat#2146): DMSO; 10 μM PSB0739 (Tocris; Cat#3983): H20; 10 μM Suramin (Sigma-Aldrich; Cat#S2671): H20; 10 μM 5-BDBD (Tocris; Cat#3579): DMSO; 10 μM {"type":"entrez-protein","attrs":{"text":"PSB12062","term_id":"1360388460","term_text":"PSB12062"}} PSB12062 (Sigma-Aldrich; Cat#SML0753): DMSO; 10 μM A438079 (Sigma-Aldrich; Cat#A9736): DMSO; 100 μM JNJ 47965567 (Tocris; Cat#5299): DMSO; 100 μM isoPPADS (Tocris; Cat#0683): H20; 100 μM oxATP (Sigma-Aldrich; Cat#A6779): H20; 50 μM BzATP (Sigma-Aldrich; Cat#B6396): H20; 50 μM FP-ZM1 (Tocris; Cat#6237): DMSO; 10 μM RAGE antagonist peptide (Tocris; Cat#6259): H20; 10 μM Pertussis toxin (Sigma-Aldrich; Cat#P7208): H20; 0.5 μg/ml Animals were treated systemically (i.p.-injections) with following inhibitors: list-behavior=unordered prefix-word= mark-type=disc max-label-size=0 Clopidogrel (Sigma-Aldrich; Cat#C0614): DMSO; 1 mg/kg; 3 consecutive days.

Techniques: Recombinant, Software, Imaging, Light Microscopy

Journal: Cell

Article Title: RESIDENT MACROPHAGES CLOAK TISSUE MICROLESIONS TO PREVENT NEUTROPHIL-DRIVEN INFLAMMATORY DAMAGE

doi: 10.1016/j.cell.2019.02.028

Figure Lengend Snippet: KEY RESOURCE TABLE

Article Snippet: Inhibitor studies Macrophages were topically pre-treated with following inhibitors at indicated concentrations: list-behavior=unordered prefix-word= mark-type=disc max-label-size=0 Apyrase (Sigma-Aldrich; Cat#A6535): H20; 10 U MRS2578 (Tocris; Cat#2146): DMSO; 10 μM PSB0739 (Tocris; Cat#3983): H20; 10 μM Suramin (Sigma-Aldrich; Cat#S2671): H20; 10 μM 5-BDBD (Tocris; Cat#3579): DMSO; 10 μM {"type":"entrez-protein","attrs":{"text":"PSB12062","term_id":"1360388460","term_text":"PSB12062"}} PSB12062 (Sigma-Aldrich; Cat#SML0753): DMSO; 10 μM A438079 (Sigma-Aldrich; Cat#A9736): DMSO; 100 μM JNJ 47965567 (Tocris; Cat#5299): DMSO; 100 μM isoPPADS (Tocris; Cat#0683): H20; 100 μM oxATP (Sigma-Aldrich; Cat#A6779): H20; 50 μM BzATP (Sigma-Aldrich; Cat#B6396): H20; 50 μM FP-ZM1 (Tocris; Cat#6237): DMSO; 10 μM RAGE antagonist peptide (Tocris; Cat#6259): H20; 10 μM Pertussis toxin (Sigma-Aldrich; Cat#P7208): H20; 0.5 μg/ml Animals were treated systemically (i.p.-injections) with following inhibitors: list-behavior=unordered prefix-word= mark-type=disc max-label-size=0 Clopidogrel (Sigma-Aldrich; Cat#C0614): DMSO; 1 mg/kg; 3 consecutive days.

Techniques: Recombinant, Software, Imaging, Light Microscopy

Journal: Cell

Article Title: RESIDENT MACROPHAGES CLOAK TISSUE MICROLESIONS TO PREVENT NEUTROPHIL-DRIVEN INFLAMMATORY DAMAGE

doi: 10.1016/j.cell.2019.02.028

Figure Lengend Snippet: KEY RESOURCE TABLE

Article Snippet: Inhibitor studies Macrophages were topically pre-treated with following inhibitors at indicated concentrations: list-behavior=unordered prefix-word= mark-type=disc max-label-size=0 Apyrase (Sigma-Aldrich; Cat#A6535): H20; 10 U MRS2578 (Tocris; Cat#2146): DMSO; 10 μM PSB0739 (Tocris; Cat#3983): H20; 10 μM Suramin (Sigma-Aldrich; Cat#S2671): H20; 10 μM 5-BDBD (Tocris; Cat#3579): DMSO; 10 μM {"type":"entrez-protein","attrs":{"text":"PSB12062","term_id":"1360388460","term_text":"PSB12062"}} PSB12062 (Sigma-Aldrich; Cat#SML0753): DMSO; 10 μM A438079 (Sigma-Aldrich; Cat#A9736): DMSO; 100 μM JNJ 47965567 (Tocris; Cat#5299): DMSO; 100 μM isoPPADS (Tocris; Cat#0683): H20; 100 μM oxATP (Sigma-Aldrich; Cat#A6779): H20; 50 μM BzATP (Sigma-Aldrich; Cat#B6396): H20; 50 μM FP-ZM1 (Tocris; Cat#6237): DMSO; 10 μM RAGE antagonist peptide (Tocris; Cat#6259): H20; 10 μM Pertussis toxin (Sigma-Aldrich; Cat#P7208): H20; 0.5 μg/ml Animals were treated systemically (i.p.-injections) with following inhibitors: list-behavior=unordered prefix-word= mark-type=disc max-label-size=0 Clopidogrel (Sigma-Aldrich; Cat#C0614): DMSO; 1 mg/kg; 3 consecutive days.

Techniques: Recombinant, Software, Imaging, Light Microscopy